BRIOTECH LIFE

MANIPULATING

BIOLOGY

Noninvasive Technology that cures Biofilm-related Prosthetic joint

infection (PJI)

BRIOTECH

LIFE

TECHNOLOGY

The technology will save lives, organs, and quality of life.

By disrupting the basic life processes of bacteria in the biofilm

using iron starvation , the technology is effective against all

types of bacteria, including antibiotic-resistant strains and

prevents unnecessary operations.

20XX BRIOTECH LIFE 2

PROBLEM

HOW COMMON ARE PJI?

It is estimated that 3,481,000

primary total knee and 572,000

primary total hip arthroplasties will

be performed annually in the US by

2030. With the increase in prosthetic

joint implantations, serious

complications of prosthetic joint

infections (PJI) of the hip and knee

is also on the rise.

(An Overview of Prosthetic Joint

Infection (PJI) Definition and

Diagnosis, By Laboratory Best

Practice blog Jasper Zheng, M.D.

and Anna Maria Romanelli, Ph.D.)

The overall cumulative PJI incidence

of 1.2% (with a higher rate in knees

than in hips) is in line with the

results of other studies (Zimmerli et

al. 2004, Pulido et al. 2008)

42,000 Knee PJI , 7000Hip PJI

EXISTING

SOLUTIONS

EXTRINSIC METHODS

OF BIOFILM

DESTRUCTION:

Can be grouped into two categories,

non-invasive and invasive methods.

Non-invasive methods include (1)

photodynamic therapy in combination

with a photosenziting substance

which triggers release of ROS, (2)

sonication, and (3) alternating

magnetic fields causing local heating

of implant surface while invasive

methods include (4) direct application

to the implant causing heating, (5)

CVCES w hich utilizes two implanted

electrodes plus the implant itself to

create a cathodic voltage causing

electrostatic repulsion and creation

of ROS, and (6) plasma treatment

utilizing floating electrode dielectric

barrier discharge.

https://www.ncbi.nlm.nih.go v/ pmc/arti

cles/PMC9854850/figure/antibiotics-

12-00054-f002/

1)Not effective

between bones.

2) Can cause

burns.

3) Can cause

burns.

4,5,6) are

invasive.

CONS OF

EXTRINSIC

METHODS OF

BIOFILM

DESTRUCTION:

COSTS OF

STANDARD CARE

FOR PAYER

Noninvasive magnetic

technology to treat biofilm

related infections by

causing iron starvation of

the bacteria.

Treatment based on our

technology can save

payer’s payments of about

1.8 BN $ per year

$1.85 billion by 2030. This

includes $753.4 million for

THA PJI and $1.1 billion for

TKA PJI, in that year.

Projected Economic Burden

of Periprosthetic Joint

Infection of the Hip and

Knee in the United States.

OUR SOLUTION

THE MARKET TARGET MARKET

Outpatient’s clinics and hospital

departments that treat PJI and other

biofilm-based infections.

THE MARKET SIZE

I n 2021, the global Prosthetic Joint

Infections market was valued at USD

482.9 million and is projected to

reach USD 1043.33 million by 2028

https://www.c rede ncere search.c om/re port/pr

osthetic-joint -infe ctions-market

THE PRODUCT

Treatment by systems based on

developed technology can save

payer of about 1.8 BN USD per

year and will help to avoid

revision surgery

MARKET AND

PRODUCT

IRON STARVATION AND BRIOTECH LIFE SOLUTION

Iron Starvation:

spatial shaking

of Fe2+ ions

could disrupt

biofilm

formation or

bacterial

growth by

preventing iron

acquisition

from the

surrounding

environment.

Iron is an

essential nutrient

for bacteria.

Iron is

transported into

the bacteria via

membrane

receptors.

Continuously

spatial shacking

of the Iron atoms

in a manner that

prevents their

proper

interaction with

the receptors or

transport

proteins, can

impede the

uptake of iron by

bacteria.

Few hours

treatment will

yields bacteria

inhibition.

During bacterial infection,

pathogen and host

compete for iron (Fe). The

inflammatory response

associat ed w ith infection

shifts Fe from the

circulation into storage,

resulting in hypof erremia

and iro n-deficient

erythr opoiesis, and

ultimately contributing to

the anemia of

inflammation.

Bacteria emplo y both

membrane-bound

transferrin receptors and

high-affinity iron-binding

proteins called

siderophores to acquire

Fe. Humans utilize the

iron-bind ing proteins

lactoferrin, transferrin,

and ferritin to move Fe

away from sites of

infection and into

storage. Synth esis and

action of these proteins

are regulated by

INTERFERING THE

NATURAL

BACTERIAL IRON UP

TAKING (CALLED

"IRON STARVATION")

WILL CAUSE BIOFILM

ERADICATION

IRON STARVATION AND BRIOTECH LIFE SOLUTION

Interfering is possible through

these 3 different types of

influence, by interrupting:

•The Ferric-Siderophore

spatial correct docking

over the membrane

receptor.

•The Fe2 natural taxing

inside the receptor

•The Fe2 natural taxing

inside the cytoplasm

Fe2 and Ferric-

Siderophore

molecules are

paramagnetic,

and are attracted

to magnets

Spatially shaking

them by

alternated

magnetic field

will prevent Iron

taxing from outer

media to the

target inside the

bacteria.

Iron starvation-

based treatments

have been explored

as a potential

strategy to disrupt

or eradicate

biofilms.

The treatme nt is

noninvasive , nor d rug

delivery, mate rials, o r

dis po sa bles use d.

(me an ing tha t it is c hip for

treatment p rovid ers and

patients a s a result)

The technolo gy is rele vant

generally to all

microorganisms

consuming Iro n for cell

divis ion.

The tw o electromagnets

(applica tors) ba se d

sys tem , w here ap plica tors

are energiz ed alte rnately

causing sh aking o f all

paramagnetic particles

inc ludin g Iro n c om plex

and F e2 atoms, in th e

patient’s treated zon e as

show n in the d raw in g:

CURRENT STATUS &

TECHNOLOGY BENEFITS

Up to date:

•Israeli Innovation Authority Grant received, Provisional patent,

Prototype 1, first petri dish trial, consulting joint surgeon Dr Amit

Sagi -Head of the joint surgery unit, biologists and microbiologist in

Volcani Center

•The treatment will be noninvasive, there is no drug delivery, no

materials or disposables used.

REQUIRED INVESTMENT

How much has been invested to date- 30K USD

A Grant from the Israeli Innovation Authority – 24K USD

The required investment for 3 years – 3 M USD

Which includes- patenting, prototyping, small and big animal

tests, medical tests, Certifications, full production file, building

marketing base, PR with key opinion leaders (kindly see full

budget file)

A BIT OF HISTORY:

OUR LIFE JOURNEY WITH MEDICAL

ELECTROMAGNETIC TECHNOLOGY AND

TREATMENTS OF INFLAMMATIONS, PAIN,

BEAUTY ETC’ IS LONG AND INTERESTING.

RECENTLY, FOLLOWING OUR NEW

DISCOVERIES ON THIS SUBJECT OUR

TEAM RECEIVED THE GRANT FROM

ISRAELI INNOVATION AUTHORITY.

A BIT OF FUTURE:

TO COMPLETE 10 PROTOTYPE DEVICES

FOR A VARIETY OF TREATMENTS FOR PJI

HIP AND KNEE INFECTIONS PATENTING

OF TECHNOLOGY (BASED ON OUR

PATENT PENDING)

TO START CLINICAL TRIALS.

FIND A STRATEGIC PARTNER FOR OUR

VISION

THE VISION:

OUR VISION IS TO DEVELOP EQUIPMENT BASED ON INVENTED TECHNOLOGY THAT WILL

PROVIDE TREATMENT FOR PJI HIP AND KNEE INFECTION IN A NON-INVASIVE WAY WITH FEWER

DRUGS AND ACCESSIBLE TO ANY PATIENT.

MEET THE TEAM

TINA WEINSTEIN

Co -f oun der ,

Ma rke tin g an d m ana ge me nt. T ina

bri ngs o ve r 20 yea rs of e xpe rie nc e in

int e r n at io nal m ar ke ting , sal es

ma nag eme nt an d oper at ions in

me dica l devic e com pan ies and

me dica l ins tit utio ns.

ITZHAK KREMIN

Co -f oun der ,

Re sea rc h a nd sc ien ce . Itz hak

bri ngs o ve r 30 yea rs of e xpe rie nc e

in m edi cal d evi ce en g ine er ing,

sc ien c e an d R &D

VADIM POLYAKOV

Co -f oun der ,

Eng inee ri ng and t ech nol ogy.

Va dim br ings ove r 30 ye ar s o f

ex per ien ce in med ica l devic e

eng ine er in g, st ra te gy and R &D.

D r . H a ya Fr i e dm a n i s a c el l

bi o l og i s t w ith vas t e x per i e n c e

in d iff e r e nt bi o log i c a l s y s te m s .

Fo r t h e p a s t 2 2 ye ar s sh e h a s

be e n a l ead s ci e n ti s t , Pr in c i p a l

In v e s t i g a t o r a nd l ab he a d an d ,

for 4 ye ar s a D ep a r tm e n t C ha i r

in t h e V o l ca n i Ce n ter .

S h e h a s p r a cti c a l la b or a t o r y as

we l l as r e s ear c h m a n a g e m e n t

ex p e r i e n c e , i n cl u di n g

su c c e s s f u l l y ob t a in i n g f u ndi n g

an d g r an t s f r om bot h d o m e st i c

an d i n te r na t i o n a l ag e nc i e s

THANK YOU

Tina Weinstein

+972 (0)533376010

tina@briotech.life

www.briotech.life

20XX PITCH DECK 13